Poisoning, Starvation, and Forensic Analysis with guest Dr Adam Stern
We are back after a short break, and we couldn't have asked for a better guest to get us back on track. Dr. Adam Stern, professor of forensic pathology at the University of Florida, joins us to discuss poisonings and starvation in the veterinary forensic setting. This is an educational and informational episode and, while it is a difficult topic, there is nothing gory about our discussion.
This topic is good for everyone, but in particular an excellent resource for veterinarians and humane agents wanting to improve their knowledge and learn about resources available to them.
Dr. Stern also is one of the founders of the International Society for Animal Forensic Sciences, an organization we highly recommend for anyone involved in animal cruelty and neglect investigations regardless of their experience or knowledge level.
Websites of importance shared on this episode:
https://animalforensics.vetmed.ufl.edu
Mentioned in this episode:
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Transcript
Hi and welcome to the Animal Welfare Junction.
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:This is your host Dr.
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:G and our music is written
and produced by Mike Sullivan.
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:I want to apologize to our
listeners because I have not put
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:anything out in about six weeks.
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:It's been crazy six weeks, but we are
back and we have a great guest today.
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:We have Dr.
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:Adam Stern, Professor of Forensic
Pathology at the University of Florida
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:and founder of the International
Society for Animal Forensic Science.
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:So I'm really honored to have
you as a guest today, Dr.
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:Stern.
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:So thank you for taking the time
and welcome to the junction.
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:Dr. Stern: Well, thanks for having me.
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:I'm excited to be here.
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:I actually listened to a couple
of your talks earlier this week.
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:Uh, so I like what you're doing
and I'm glad to be a part of it.
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:DrG: Thank you.
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:Thank you so much.
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:So let's start, you know, I know
a little bit about you, but our
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:listeners may not know who you are.
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:So let's start by letting our
listeners know about how you got
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:started and the path that brought
you to where you are today.
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:Dr. Stern: Sure.
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:Well, I have a non traditional path of
sort of how I became a veterinarian.
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:You know, we talked to most veterinarians,
veterinary students, and they all
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:wanted to be a veterinarian since
they were born or two years old.
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:That wasn't my dream, right?
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:I, I went to, uh, college and I
actually, when I was there, uh,
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:was interested in counterterrorism.
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:That was, that was a
thing that I wanted to do.
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:Um, but my college advisors didn't
really, um, think that was a, a good idea.
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:Um, and so they really pushed me in
another direction and, and, and that's
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:how I figured out I, I, I like biology,
veterinary medicine sounded interesting.
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:Um, and, and so that's how I got there.
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:I just, You know, never worked in the
veterinarian's office until till college.
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:So I didn't really know
what I was getting into.
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:And it wasn't until, you know,
going through vet school, you're
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:like, well, I like horses, but
I don't want to work on horses.
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:Zoo animals are cool, but I don't
want to work on zoo animals.
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:Um, and so I made my way to, um, so
the, the, the back services, so like
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:radiology, pathology, things like that.
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:And then I decided pathology
was really where I wanted to go.
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:So I kind of got there in a,
in a non traditional direction.
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:Uh, but you know, I look back at it and
I'm like, I guess I made all the right
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:choices to get to where I am today.
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:So I'm not complaining.
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:DrG: Yeah.
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:I think that everything kind
of happens for a reason, right?
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:Hey, because I think that, I mean,
you're doing great things there.
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:And I joke with my staff that
you're going to be my boss someday.
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:So in like four or five years, watch
out for my resume because I'm coming.
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:I just really, I just, uh, you know,
and we're going to talk a little bit
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:about the services that you guys do, but
I have used your services and I think
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:it's, it's a great benefit, not just
to veterinarians in forensics, but just
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:humane officers, animal control officers.
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:law enforcement.
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:So, um, yeah.
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:So thank you for sharing
what you have done.
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:And now we'll start with our topic.
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:So we're going to be talking about
poisonings and starvation that
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:can be somewhat difficult topics.
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:So for our listeners, if this is
hard for you to listen, hit the pause
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:button, focus on something else for
a bit, and then you can come back.
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:Um, let's start with
talking about poisoning.
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:So in my experience, most pet owners,
when I was in general practice, most
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:people that came in claiming that the
neighbor killed the dog or the cat
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:and that it was poison, very few of
those actually, I can't even think
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:of any of them that were true that
the that the neighbor did poison it.
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:Um, what is your
experience with with that?
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:Dr. Stern: So, yeah, Poisoning
cases in general, they, they kind of
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:present non specifically and sometimes
they're not poisoning at all, right?
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:Like you said, a lot of them are, are not.
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:There's a natural process, but it kind
of unexpected demise of the animal.
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:Um, but you still have to look
into it and, and, you know,
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:consider it as a, a differential.
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:Uh, in my experience, uh, we actually
are, are doing a lot of retrospective
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:work and we have found that the
neighbor sometimes does do it.
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:Uh, you know, it really does happen.
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:Uh, but again, that's not,
that's not the commonality.
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:A lot of times it's,
it's, it's something else.
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:Um, but they're, they're really
difficult cases to handle.
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:You really have to invest a lot of
time and honestly, a lot of money,
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:um, to really figure out, you know,
what the poison is, if it actually
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:was a poison in the first place.
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:DrG: Yeah, most of the poisonings that
I saw were what we thought primarily
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:accidental, right, like rat poison or
ethylene glycol, that kind of stuff, but
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:one of the things that I think people
have a misconception, and it's probably
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:because of the CSI effect, is that they
think that something is poison, and then
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:just Send blood to the lab and see what,
you know, they expect to see the graph
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:and say, yeah, this is what happened.
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:So, what is the, what is the truth as far
as what it actually takes to find out what
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:poisons may be in a, in a dog or a cat?
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:Dr. Stern: Yeah, so you're,
you're absolutely right.
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:That CSI effect, you know, I always, I
always talk about, you know, you have this
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:45 minutes to, you know, watch that, that
detective show, that crime scene show.
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:And in about 30 seconds, they put
the sample in the machine, it turned
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:around and it prints out and voila,
you got your, you got your answer.
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:Uh, so far from the truth.
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:It's really just not even funny.
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:Um, when we look for, for toxins.
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:Uh, there's a plethora of different, um,
analytical techniques that one can use.
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:We have GCMS, LCMS, we have, uh,
immunoassays for screening these
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:cases, uh, but there is no one test
that fits all poisons and toxins.
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:Uh, heavy metals are going to be
identified differently than say,
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:uh, ethylene glycol, which will
be different than organophosphate.
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:So, uh, we really have to consider.
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:Uh, so much more than just the, here's
the blood sample or a blood might not
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:be, uh, the best, um, substance to
even test, uh, you know, if it's a
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:more of a chronic intoxication, um,
in the live animal, you're probably
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:gonna be looking at urine, right?
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:A little blood test.
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:The toxins probably not
circulating in the blood anymore.
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:It's probably been
deposited in all the organs.
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:And so we have to really consider
when the intoxication happened.
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:Um, you know, same thing.
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:If it's per acute, animal literally
eat something and drops dead.
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:Um, we're not gonna be looking at urine.
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:We really want to look at the
vomit or the stomach content.
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:So again, you have to think
about the clinical picture.
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:Uh, in my case, the autopsy findings
and then, you know, what our
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:list is of differentials for the
toxin and then the test itself.
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:So lots, lots more than just,
you know, 30 seconds on TV.
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:DrG: Yeah, no, absolutely.
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:And what, what is the importance as far
as like the history that people give you?
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:Dr. Stern: So the history,
it's, it's important because
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:say like the pet owner, right?
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:They, they know their animals really well.
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:Um, and I had a case recently
where, uh, the owner basically said
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:I was able to pet the cat and he
said, that is completely abnormal.
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:That cat would never let me touch it.
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:And now I can pet it.
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:And then unfortunately it,
it died shortly thereafter.
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:Um, but even subtle signs like that
can be mean a lot, doesn't say what
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:it was, but it kinds of starts to give
us clues like this animal is not doing
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:well or, you know, abnormalities.
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:But the history is important, but you
also have to look at the clinical picture.
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:The history can be kind of, you
know, kind of saying, Oh, it's a
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:screaming, uh, a malicious poisoning
case or something like that.
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:But then when you start to actually
look at the animal, you're like, well,
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:no, that's really not what's going on.
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:So use that history for sort of initial
guidance, but then you have to let our
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:science, veterinary medicine, uh, really
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:put us in that direction of, of
which fork in the road, which
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:differentials we need to consider.
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:DrG: Yeah, I had, uh, I had a case
where a lady brought in a cat and
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:she said that the neighbor poisoned
the cat because the cat was fine
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:and then the cat was stumbling
and acting drunk and then it died.
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:And when she brought it in on necropsy,
it actually had a urethral obstruction.
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:So, it was blocked and the changes that it
underwent from the toxins are very similar
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:to what some people would expect that an
animal that was poisoned would look like.
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:So, can you share some of the cases
that you have worked on with toxins
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:and how you have worked through them?
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:Dr. Stern: Yeah, sure.
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:I mean, the, the, the case that
you're presenting right there,
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:the urethral obstruction, I mean,
that, that history, it's obviously
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:ethylene glycol, antifreeze ingestion.
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:Um, and we see, we see a lot of that.
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:Um, so we, we actually do a lot of
research and some of the research we
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:do is on, on how to, um, more readily
document those cases and, um, actually
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:doing, uh, postmortem chemistry
to, um, estimate antemortem blood
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:values for like BUN and creatinine.
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:Um, and so we're doing all of that, but
that's, that's, I would say, um, for
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:us, for, um, probably the most common
one that we see, um, readily available
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:for, for people to, um, have at their
house, whether it's intentional or
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:accidental, that's out of the realm of
the veterinary forensic pathologist.
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:We just say, this is what it is.
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:And then the investigator, you know, Goes
and does their part of the investigation.
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:So they're going to be the ones who
are going to be looking more into that.
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:Um, but that's quite common.
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:That's actually really easy
for us to, to diagnose.
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:Um, and we, we do that a lot.
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:Um, then you get into some of the
things like the anticoagulants
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:again, something that you would see.
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:Then you get into more difficult
ones, such as the illicit drugs.
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:Um, you know, whether an animal
is fed, um, a laced meatball, you
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:know, some sort of food material.
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:Um, once we start seeing things, we
asked the clinician, the owner, uh,
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:what What do you feed the animal?
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:We always ask those sorts of questions on
our, on our submission forms because we
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:look at the stomach contents and you're
like, well, you say this is what you
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:fed, but you have this in your stomach.
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:Um, so that doesn't match.
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:And so that might be a little
clue, uh, to go down some of these
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:more sort of acute per acute.
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:Intoxication.
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:And so, uh, illicit drugs would be one.
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:Um, uh, pesticides,
insecticides would be another.
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:Typically, they cause rapid death.
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:Um, the animal doesn't linger on for days.
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:Typically, they eat it.
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:Um, and they get pretty sick really fast
and you just find them deceased in their
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:backyard or wherever they would have been.
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:Um, so, you know, I think we see
a lot of different things but
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:uh, again, tailoring what we see.
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:With our sciences, uh, really help us.
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:DrG: I know that also in clinical practice
when animals, especially dogs, would have
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:like vomiting or diarrhea and the owner
was not sure, people would always blame
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:it on the control, like weed control and,
uh, fertilizer and that kind of stuff.
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:So is there any truth as far as the
toxicity level of just stuff that's
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:spread out in the yard to treat the yard?
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:Dr. Stern: Yeah, so we've, we've, we've
worked on a number of those, those
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:kinds of cases where there's some lawn
stuff put down and then the dog is sick
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:shortly thereafter, or, you know, within
a couple of days, um, and, and really
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:to my experience is a lot of those
animals have some other disease that was.
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:Subclinical asymptomatic and it started
to rear its its ugly head at that point.
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:Um, and then there's others where
unfortunately um, you know, we we don't
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:find any Gross findings at autopsy.
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:We, we do a histology looking at the
tissues and we don't see anything.
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:And then we offer up doing
some toxicology analysis.
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:And, um, again, because it comes
down to finances, um, people
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:just don't go that next step.
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:And so probably some of those cases do
have, um, some validity, but I think a
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:lot of times it comes down to finances.
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:And it just doesn't get
fully, uh, worked up.
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:DrG: Another thing that people
get concerned about is what
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:you brought up as far as asking
people what they feed their dog.
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:It's about potential toxins in the food.
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:Um, how often, how often do you see that?
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:Dr. Stern: Uh, well, in our
lab, we actually, we don't
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:get a lot of those cases.
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:Um, they, they seem to, there's different
mechanisms, um, reporting these to the,
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:the, the federal government where they go
through, uh, some of those other channels.
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:Um, we've seen a few.
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:A lot of times it's going to be more of
the Bacterial contamination might be in
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:there, maybe a salmonella or, uh, some
other bacteria, but we honestly just
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:don't, we just don't see a lot of them.
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:DrG: If somebody is concerned,
especially like a veterinarian, if
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:somebody thinks that an animal has
been, uh, poisoned, what are going
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:to be the best samples to take
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:and submit and how they're
going to go about doing that?
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:Dr. Stern: Yeah.
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:So you, you have a difference between
the live animal and deceased animal,
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:uh, because the samples we can get are,
are, are obviously vastly different.
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:Um, but you have to think about, is
it an acute intoxication or is it
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:more of a chronic, did it happen?
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:You know, days ago.
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:Uh, so with the acute intoxications,
and this is not an exhaustive list,
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:you have to think about things, uh,
where the toxins going to be, so vomit.
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:Okay, that's going to be a good place.
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:Um, gastric contents, uh, goes
along with the vomit, uh, blood,
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:because if you inject, ingest it,
it's going to go into circulation.
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:And so we want to get
things that are earlier on.
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:Um, if it's cutaneous contact, like
something is put on the animal,
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:then, then obviously for skin from
that area would be, would be useful.
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:Um, In the more chronic cases, again,
it's going to be more of what's in the
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:organs and sort of downstream to that.
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:So, uh, urine for a live animal,
even feces, sometimes, uh, it's
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:going to be excreted in the
feces as well as the urine.
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:So that's potentially a way to get it.
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:Uh, with the autopsy, it's sort of
the same, the same thought process.
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:If it's acute, we go for the, the
bloods, the, the stomach contents,
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:um, and even liver, um, it's cause
it's going to be likely metabolized in
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:the liver or in the kidneys, and then
for the more chronic, we do organs
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:as well as the urine.
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:So it kind of, again,
just kind of depends.
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:Um, but then you also want to collect
other samples because maybe that drug, um,
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:or toxin is maybe, um, likes a lot of fat.
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:And so we always collect
some sort of fatty tissues.
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:Um, and really just a wide array of
tissues just in case, um, we were
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:dealing with some sort of odd situation.
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:DrG: I just finished the Forensic
Science Masters at the University of
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:Florida, and one of the things that
they tell us also about collection is
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:the vitreous fluid inside of the eye.
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:Is that something that is
done in animals as well?
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:Dr. Stern: So yeah, so we do do that.
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:It's good for like in livestock,
for example, testing for nitrates
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:and nitrites for cattle, sort of
the acute intoxication for them.
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:So yes, we do it.
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:Uh, we can detect a number
of different drugs in it.
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:Um, even, uh, looking at the human side,
they use vitreous, uh, for a bunch of
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:different drugs, so that is definitely
something that you can collect and
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:in our lab, we use it for vitreous
chemistry, um, which I kind of alluded
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:to earlier with, uh, the antifreeze
cases, um, because you can look at
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:things such as vitreous urea nitrogen,
you could look at the electrolytes
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:a little bit, um, some just go up.
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:With post mortem change, so like
potassium, so it's not really going
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:to help you to estimate what it
was in a live animal, um, and even
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:glucose levels, uh, so we can identify
theoretically, uh, a diabetic animal
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:with a massively elevated glucose levels.
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:So, um, you can use it for toxins,
but you can also use it just for sort
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:of general, um, health of the animal.
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:DrG: Is there any, um, time limit or time
frame that the samples have to be done
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:before you cannot get any information.
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:Or is the information stay there
for a really long period of time?
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:Dr. Stern: So that's a,
it's a great question.
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:Um, and it depends.
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:Uh, some, some compounds will
break down pretty quickly.
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:Their half lives are pretty short
where others, they last forever.
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:Um, and I don't mean literally forever.
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:That breakdown takes
them a very long time.
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:Uh, there was a study looking
at, uh, panel Barbara tall.
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:And they were looking at
composting of, of pigs.
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:And there was also, I believe, a study
in horses, and they found that that
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:stuff lasted for years, um, lasted in the
decomposed remains lasted in the soil.
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:So some of these toxins, uh, potentially
can be there for, for really long times.
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:And we see the issues when, um,
maybe a dog digs up a buried horse
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:carcass and gets intoxicated.
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:So that we could detect really easily
in, in pretty decomposed remains
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:where some other toxins, um, you
have to get to them much faster.
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:Like ethylene glycol.
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:Um, you need to get that really early
in the phase because it's going to be
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:metabolized and excreted and you're
just not going to find it in the body.
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:DrG: Is there a cool case that you
have worked recently of toxicity
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:that you can share with us?
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:Dr. Stern: um honestly, I I
really like the antifreeze cases.
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:Um Not because they're they're
relatively straightforward.
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:I just think they are um one of these
cases where you know, you get the
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:accidents, but then There are truly In
here in Florida, a lot of malicious cases.
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:Um, and so being able to identify and
document those cases, um, it's really
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:rewarding because there's, there's
a high chance that the end result
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:will result in a prosecution if,
if it's determined to be malicious.
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:Um, and so it's really straightforward.
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:Easy to, um, present that to a court,
to a jury, um, for them to understand.
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:I like the, the challenges though,
of some of these unusual cases like
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:organophosphates, um, and the carbamates,
uh, because those, those cases, um, you
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:do the autopsy and you just, you just
don't see, you just don't see anything.
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:There's really no pathology.
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:And so you have to start that
sort of treasure hunt of find
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:that toxin and find that poison.
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:Um, they're expensive, uh, to, to
do that because you, you typically,
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:you're like, well, what do I do?
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:And so you could do a shotgun approach
and just kind of just do, um, some
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:random testing, or you can just
start to go a bit more specific.
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:Um, and so.
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:It'll depend on the case, obviously.
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:But if you have three, four or five
animals who, um, all of a sudden
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:they're happy and healthy and then
they're dead, um, then, you know,
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:the, the stakes are kind of a bit
higher, uh, because it's, you know,
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:definitely, um, screaming poison,
uh, in, in a lot of those cases, so.
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:You know, you can do that.
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:And then, uh, barbiturate toxicity is
also something that really interests me.
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:And we are doing, uh, some research,
um, not only for deceased animals, but
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:also, um, living animals, and we're,
we're trying to develop, um, or work
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:up a way, um, to diagnose it in the
live animals so that they can get,
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:uh, a more timely diagnosis and, uh.
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:Tailored treatment to that.
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:Um, so a lot of it's just
gonna be symptomatic care.
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:Uh, but kind of help with the
getting that diagnosis a bit quicker.
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:DrG: So let's jump into starvation cases.
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:Um, I've had the opportunity to work
with a couple of starvation cases that
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:actually resulted in the death of the
animal, but most of the starvation
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:cases are going to be like neglect,
cruelty, hoarding cases where the
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:animals are still alive and you kind of
manage those really, really different.
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:So let's start with the live animals.
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:What, what determines if an animal has
been starved as far as the, the findings,
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:the physical examination findings, the
body condition, that kind of stuff.
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:Dr. Stern: Yeah.
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:So, so obviously I'm, I'm the
pathologist, so I don't treat
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:things that have a heartbeat, right.
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:Uh, uh, but.
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:You know, I mean, they're, they're
going to look the same, the, the,
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:they're going to be obviously emaciated.
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:And it's really important for you to do
your, your body condition scores and your
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:muscle scoring, uh, because these animals
are going to be losing fat, uh, initially,
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:um, and really only in the end does that,
does that protein catabolism really.
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:Um, go into high gear.
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:Um, but besides that, I think the,
the biggest thing is it's actually
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:on, it's on the investigator really
at the very beginning, um, to be sure
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:that, you know, at the scene, um, you,
you capture obviously that scene with
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:photography and all that, but don't
just go and give the dog a treat.
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:Um, you know, don't just give them some
water, uh, because you have to be sure
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:you, you get that on video, you know, the
first time to get food, the first time
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:to get water, um, so that you can show,
look, Hey, I just grabbed this stuff
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:out of my truck and the dog just ate it.
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:Um, because a lot of times you see,
um, in these cases, the, uh, defense
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:is always, well, I tried feeding them.
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:Um, I gave them.
368
:All this great high quality food, or, you
know, this crap food or whatever it might
369
:be, and the dog just wouldn't eat it.
370
:But now we have on video, look, we
just randomly gave a small amount
371
:of food and they ate it ravenously.
372
:So I think that's really important
and actually really helpful
373
:information for the veterinarian.
374
:Because in the, the diagnosis
of starvation is really
375
:a diagnosis of exclusion.
376
:Yeah, you really go down the
fact that, look, there's, there's
377
:nothing else wrong with them.
378
:These animals are, do not exhibit
cachexia, which is, you know,
379
:that, that same sort of emaciated
condition, but for very different
380
:reasons, they are, they're sick.
381
:They have a systemic disease or even one
organ is affected, but the animal is is
382
:really sick, like chronic renal disease.
383
:So I think, um, really, with those cases,
it's the job of the veterinarian to just
384
:prove that there's no other disease.
385
:So, you know, doing.
386
:Uh, blood work, fecal exams, and yes,
you're probably going to find some
387
:parasites in them, uh, because of
the conditions, but it's not going to
388
:typically rise to that level unless
you have a massive parasite infection.
389
:Um, so doing your basic workup, um, and,
you know, getting rid of differentials
390
:will really back you into the
starvation by exogenous circumstances.
391
:DrG: Yeah, that's a great point.
392
:There was, uh, several years ago,
there was a humane officer, it was
393
:well over 80 dogs that they rescued
from, uh, from a starvation case.
394
:And, well, a neglect case.
395
:And I want to say it was a dog
fighting case, but I don't remember.
396
:But the owner said that the dogs
could not gain weight because
397
:they had a genetic problem.
398
:So, you know, that was
kind of like the defense.
399
:So the officer, the investigative
officer called me and he's like, how
400
:am I going to prove that these dogs
don't have this genetic condition?
401
:And I told him, just feed them, just
402
:Dr. Stern: feed them
403
:DrG: and then see if they gain weight.
404
:And if they gain weight,
then it's not genetic.
405
:And he, and it's such a simple answer
that you just don't think about.
406
:And they fed them and the dogs fattened
up and then that was the end of that.
407
:So it was a pretty simple
solution to, uh, what, what
408
:seemed to be a difficult question.
409
:Dr. Stern: Yeah, and that, and that
brings up another good point is you're
410
:going to want to periodically weigh
them, periodically take photographs of
411
:them, because if you have this dog that
comes in, say on the Purina scale, it's
412
:a one out of nine, um, which is important
to always say what scale you're using.
413
:So, um, people can actually go
back and look and don't just say
414
:it's a, a three out of five and
you know, what scale are you using?
415
:I have no idea.
416
:Um, cause all scales are
really not created equal.
417
:Um, but weigh them, take those
photographs of them to show that
418
:change, you know, over the weeks of
recovery because they're not going
419
:to eat and get better in two days.
420
:It's going to be this long, long
process, but you're going to see
421
:that that progressive change.
422
:, DrG: I just recently shared a case
that that I was working on and
423
:a lot of people got gut reaction
was, Oh my God, feed those dogs.
424
:And people don't understand that
you can't just throw food at them,
425
:that there is the whole refeeding
syndrome and you can actually cause
426
:more problems than not, if you start
feeding these, these starved animals,
427
:Dr. Stern: Yep, exactly true.
428
:Yeah.
429
:You can't just say, here's your, your,
I'm just going to make up a number of
430
:your 2000 calorie diet, um, because you,
you're, you haven't had food for so long,
431
:you'll probably end up killing them.
432
:So.
433
:You got to do it slowly.
434
:DrG: Let's go more on your side on the
animals that do die from, from starvation.
435
:So what are going to be the most
important things that you're
436
:looking for during that necropsy?
437
:Dr. Stern: So it's, it's kind of similar.
438
:So again, we're going to look at, you
know, their fat stores or lack of fat.
439
:Um, we'll look at their,
their muscle, see how.
440
:Poorly or well muscled,
they are that sort of thing.
441
:Um, but then we're going
to go organ by organ.
442
:Um, every system will get examined for
Um evidence of disease, uh, you know
443
:these organs with starvation they'll
atrophy So yes, they'll be smaller,
444
:but we want to show how there's
no, you know, systemic inflammatory
445
:disease or cancer, renal failure.
446
:Something like that to explain why this
one animal looks the way it does Um,
447
:you know, so we're gonna, we're gonna
be doing all that, looking at really
448
:the entire body, um, but with the
advantage of being able to look at the
449
:organs themselves and not just looking
at the blood work for organ function.
450
:DrG: When somebody is asking about
cases of starvation, they want
451
:to know about the cause of death.
452
:And then they also want to
know about the time of death.
453
:So what are the ways that we can determine
time of death on these starvation cases?
454
:Dr. Stern: So, time of death is
just a whole other ball game.
455
:Um, there's different ways to, to try to
estimate it and in veterinary medicine,
456
:um, and even in human medicine, it's,
it's not an exact science, um, you know,
457
:there's always a new method that someone
comes out with, um, but a lot of it is
458
:going to be very specific to a certain
location or even research, um, you know,
459
:very specific parameters, um, and, and,
and, um, For me, it's entomology would
460
:be it's the well validated, um, and
so we can use the insects, you know,
461
:if we have some, some maggots, some
pupa, um, on the body, but, or, or even
462
:around the body, because we have to
look there as well, that's going to help
463
:us a lot, giving us sort of a minimum
time of colonization for those insects,
464
:and so that would Move over to, um,
estimate in that post mortem interval.
465
:So that's really, um, just one way, but
there's a lot of research out there.
466
:There was, um, I'm working with
a student right now and she
467
:tried to use, uh, some immune
immunohistochemistry, some flow cytometry.
468
:Um, but there's all sorts of things
we talked about vitreous humor.
469
:Uh, so looking at potassium levels in
the eye, um, you know, so there's lots
470
:of different ways, but a lot of them are,
471
:especially in the animal realm,
where they're really in its infancy,
472
:um, I'm trying to work these up.
473
:Uh, so that's why for me, I fall
back to entomology, uh, especially
474
:when I have any insect activity
going to be probably very helpful.
475
:DrG: And I have to say, I hate insects and
that I'm scared of insects, but I learned
476
:to love insects from my entomology class
during my veterinary forensics training.
477
:Um, and, and yeah, it's like people
think, again, CSI effect, that
478
:somebody just looks and some larva
out in the field and says, this
479
:has been dead for about 12 days.
480
:And that's not how that works.
481
:I learned that the hard way because during
my class, I remember at the very end,
482
:we had to do a time of death estimation.
483
:And my result was something
like 54 to 800 days.
484
:And I was like, clearly
I'm doing something wrong.
485
:And I just redid it so many times.
486
:And yeah, it was like 54 to 800 days.
487
:But it's not as specific as
people, as people may think.
488
:Dr. Stern: Oh, yeah.
489
:Um, and I just and just to go back to the
starvation thing for a second, because
490
:I wanted to say, you know, for for me,
the the animal is no longer with us.
491
:So it's deceased.
492
:And so I don't have that luxury of
feeding them like we talked about in
493
:the live animals, see what they do.
494
:So one of the things we actually
looked for is foreign material.
495
:Um, in their stomachs and in their
intestines and in whatever fecal
496
:matter they have, because these
animals are hungry, um, they're
497
:going to eat anything they can.
498
:So non nutritious substances, styrofoam,
plastic, um, anything like that is
499
:going to be very valuable, uh, for us
to say, look, they, they had some sort
500
:of appetite, they clearly can swallow.
501
:And so it gives us a little more
oomph to being able to, to sort of
502
:back our way into that starvation.
503
:So in
504
:DrG: the two cases that, that I examined,
um, and one of them, the owner said
505
:that the dog had parvo and it died.
506
:Um, and then the, the other
one there was proof that they
507
:had actually been fighting.
508
:It was a domestic violence situation
and they literally starved the dog.
509
:Um.
510
:But one of the things that I submitted
was femur for bone marrow analysis.
511
:So, can you explain what, what
is that, uh, and what's the
512
:information that we can get from it?
513
:Dr. Stern: Yeah, so, so the femur
analysis, and you're looking specifically
514
:at percentage of bone marrow fat.
515
:And so, for me, my viewpoint on that
is that's really an adjunct, uh, to
516
:Your autopsy, um, your autopsy is
going to be looking at all the systems
517
:and it's looking at fat stores.
518
:And one of the things that we
routinely look at, whether or not
519
:you're a starvation case or not, is
what does your bone marrow look like?
520
:Um, so in an adult animal,
bone marrow is mostly fat.
521
:Okay.
522
:Um, I look at bone marrow.
523
:So like a can of dog food or
a can of cat food, it is some
524
:basic macronutrients in there.
525
:You have water, which is moisture.
526
:You have carbohydrates, fats, and
protein basically is what you got.
527
:And then there's ash, which
is a very small percentage.
528
:Now in bone marrow.
529
:Um, Adult, mostly fat.
530
:There's no carbohydrates.
531
:There's very little protein.
532
:Um, there's some ash and
very little moisture.
533
:And so, um, people use that, um, to sort
of quantify the percentage of fat, which.
534
:For me, I have already
looked at bone marrow.
535
:I've already said there's
gelatinous change to the marrow.
536
:I've looked at it under a
microscope to show there's no fat.
537
:And, and really the fat
analysis, all it's doing for me,
538
:it's just giving me a number.
539
:It's, it's more objective.
540
:Um, and so we're going to give a number
to my subjective, uh, interpretation.
541
:And so that's really what,
what you can use it for.
542
:You can't use it to diagnose starvation.
543
:Um, because it's, it's
not specific to a cause.
544
:It just says you have very little fat.
545
:Um, and then you also do have to
be careful, though, that there
546
:will be cases where you have
very little fat in your marrow.
547
:Um, but you didn't starve to death.
548
:So you take a dog who has leukemia, right?
549
:The neoplastic cells are actually going
to displace and um, You know, efface the
550
:fat, so that animal is actually going
to have very low fat in its marrow.
551
:And so if you looked at that
just by itself, you'd end
552
:up with the wrong diagnosis.
553
:Um, so any tests that you do, regardless
if it's a fat test or a blood test or
554
:a toxin test, you have to look at it
in totality and not just as a silo by
555
:itself, because it's only going to give
you a small piece of information that
556
:you have to look at it all together.
557
:Um, and so, uh, I get asked a lot, you
know, if I just send you a bone, well,
558
:will you tell me anything about it?
559
:I'm like, I could, we could tell you
that, but we have to look at it in
560
:the context of, of everything else.
561
:DrG: So in the, in the cases
that, that we were working, it
562
:was kind of like that, right?
563
:The animals were starting to decompose.
564
:So is, are they missing,
they had no subcutaneous fat.
565
:So we were thinking that
that's what was going on.
566
:The stomach, uh, one of the things
that I found in the stomach and was
567
:that there was like a sludge in there.
568
:And at first I thought that it was food,
but then discussing with other people,
569
:I was informed that they will create
somewhat of a sludge in the stomach,
570
:um, just from the acids and that kind
of stuff that can be confused with food.
571
:So can you tell us a
little bit about that?
572
:Dr. Stern: Yeah, so the, these animals
sometimes have gastric ulcers, so
573
:they'll be bleeding in there, and so
that mixed with the gastric contents,
574
:the acids and fluids, um, can get
that more sludgy appearance to it.
575
:Um, so that's also one thing we look
for is do we have gastric ulcers, um,
576
:because they might have some GI bleeding.
577
:DrG: So overall, I mean, clearly it's
going to be easier if there's a starvation
578
:case and somebody wants to consult with
you for you to do the complete necropsy
579
:rather than look at different samples.
580
:Is there something, though, if that is
not possible, what kind of things can
581
:you guide veterinarians in trying to
determine if there was a starvation case?
582
:Dr. Stern: Yeah, so I think if,
you know, consulting on a case,
583
:um, basically if someone else is
doing the autopsy, it's always, you
584
:know, look at everything, right?
585
:Look at, at everything from the, you
know, the tip of the nose, the tail
586
:to the feet and everything in between.
587
:Um, and then work with your pathologist
because they're the ones who can look
588
:at the tissues under the microscope.
589
:So we can say, yes, we
don't have any disease here.
590
:You have all of your your
data that you've collected.
591
:And it's a really great way to a
have more than one person look at a
592
:case because it's always better to
have consults and other specialists
593
:to help back up your claims, um,
and sort of get us to that point.
594
:So, um, you know, if you think it's a
starvation case and all you submit to
595
:someone to look at under the microscope
is lung, that's not very helpful.
596
:Um, so really, you know, give us a
full set of tissues so we can actually
597
:give you some powerful data that again,
combining with yours, um, really.
598
:Again, helps, helps the science
show what was or was not going on.
599
:DrG: How does the University of Florida
help with these cases and what is
600
:the process that humane officers or
veterinarians, uh, take to send you
601
:samples or consult with you guys?
602
:Dr. Stern: Yeah, so we, we
work with with everyone, right?
603
:We'll work with the, uh, the investigator.
604
:So whether it's law enforcement,
animal control, animal services,
605
:depending on whose jurisdiction it is
and, and who does what in that area,
606
:uh, veterinarians, uh, attorneys.
607
:Uh, and even the pet owner will,
will work directly with them, uh,
608
:through their veterinarian to sort
of help them through the process.
609
:But, uh, we, we pretty
much do everything here.
610
:We do obviously the forensic autopsies.
611
:Um, we have two, uh, fellows of
veterinary forensic pathology here.
612
:Um, and so there's going to be
typically, uh, one or two sets of eyes on
613
:on every case that comes to our lab.
614
:Um, but we do, uh, whether it's in
house or in consultation with other
615
:institutions or laboratories, um,
we do, uh, some forensic toxicology.
616
:Uh, entomology, DNA analysis, uh, we
even, um, work with people who do, uh,
617
:photogrammetry and so that's for, uh,
often for wildlife, you know, based
618
:on photographs, how is that image, uh,
whatever in that image, if it's a deer,
619
:for example, is that the same one that's
You know, we have a dead on the road or
620
:somebody poached that sort of thing so
we can kind of match the photographs.
621
:We do, , crime scene response.
622
:So we'll go out there and help
with the investigators, um,
623
:whether it's documenting the scene
or helping with an excavation.
624
:Um, and even if we have live
animals, although I don't practice
625
:live animal medicine, um, we help
with logistics and we help, um,
626
:identify people who can help them.
627
:Uh, so if we can't do it, we will
100 percent say, you know, we're
628
:not the ones for that, but you
can go, um, to this person and
629
:they'll, they'll totally help you.
630
:DrG: Cool.
631
:And how, what's the website
or how can people get ahold of
632
:you guys to, to do a request?
633
:Dr. Stern: Yeah.
634
:So actually we have a
new website rolling out.
635
:Um, we literally hit the go button the
other day, so the site, uh, should go
636
:live very soon, but it's, it's actually,
I'll give you the new address it's animal
637
:forensics.vetmed.ufl.edu and that will
take everyone to everything that we do um,
638
:whether it's the services the the research
or even the teaching and outreach that we
639
:do so it's going to be a one stop shop Um,
we have several other websites But we're
640
:going to close them all down and and kind
of direct them all to this this one site
641
:Which we're really excited about this
642
:DrG: Excellent.
643
:And we'll make sure to share that
because it's a great resource.
644
:So finally, I want to close by
talking about the International
645
:Society of Animal Forensic Sciences.
646
:So what led you to
found this organization?
647
:Dr. Stern: So, um, I founded
this organization with, with two
648
:other, um, individuals, uh, Dr.
649
:Jason Bird and Adam Leith.
650
:And it is Geared towards the investigator,
um, because they, they, you know, they
651
:have a hard job, uh, going out there
and investigating all these cases.
652
:And sometimes the investigator, um,
really doesn't have that background
653
:in animal cruelty investigations
and forensic investigations.
654
:And so we thought this was a, a, a
great way and, and something that
655
:wasn't really out there, um, to
help, to help this subset of, of.
656
:Of people.
657
:Um, and so we, we founded it and
it's, it's taken off really well.
658
:Um, we, um, have members from all
the habitable, uh, continents.
659
:Um, so if there's anyone working in
Antarctica and they, they, they want
660
:to join, they have a free membership.
661
:Um, you know, as long as they're
dealing with something, um, animal
662
:forensic related, um, you know,
there's, there's, you know, always.
663
:Great things that people do out there.
664
:Um, but we really wanted to, um,
develop an organization that was
665
:really geared towards the investigator.
666
:, and, , in doing so we've, have
a great group of people.
667
:Um, we have actually a
lot of veterinarians.
668
:Uh, we have some attorneys, law
enforcement, animal control.
669
:Um, we really have everybody who, who.
670
:It's all the walks of life that are
involved in these investigations, and
671
:it's just a great way to, um, sort of.
672
:Help to, to build one's, um, education,
whether it's, um, foundational,
673
:like they just started, or they
are a seasoned investigator who
674
:have been doing this for years.
675
:There's still, there's
always new things to learn.
676
:Like, there's always a new technique that
maybe they didn't think about before.
677
:DrG: One of the things that I have
really enjoyed is the coffee talks.
678
:Like, I feel that there's a lot
of interaction, uh, and, you know,
679
:it's kind of nice having something
like that for individuals that
680
:are interested in forensics.
681
:, what can people expect
out of these coffee talks?
682
:Dr. Stern: Yeah, so, look, the
Animal Forensics coffee talks,
683
:um, We we really like them.
684
:Um, and the one thing I like about them
is they're short the talks are 15 20
685
:minutes Um, it's a half hour block, right?
686
:Um, we get lots of questions and stuff
like that in the end as you've seen so
687
:like it goes it goes the whole time But
the whole point of them is to teach people
688
:like one thing, like a lesson learned,
like this actually helped my case.
689
:Um, it's not there to be, you know, uh,
a 60 minute lecture on a huge topic.
690
:Like our upcoming one is, is
specifically looking at chemical
691
:enhancement of, of blood.
692
:So blue star and luminol and really
just compare and contrast them.
693
:Um, and that's all that's going to be.
694
:It's not going to.
695
:You know, be the, this
whole drawn out thing.
696
:It's it's, it's to the point.
697
:Um, but they've been great.
698
:And the reason why I wanted to do
them really short is the investigator.
699
:Honestly, they're busy.
700
:The veterinarian, like myself,
we're, we're super busy.
701
:And so being able to set aside an
hour or two hours is really hard,
702
:but for 20 minutes, 25 minutes, they
can sit in their vehicle, um, listen.
703
:Uh, maybe just kind of do some basic
paperwork, um, and, and learn something.
704
:And so that's, that's our whole point.
705
:Um, and we're about to, right
now we're doing it once a month.
706
:We're about to do it twice a month.
707
:Um, and so, I mean, You
have so many topics.
708
:We're going to do like mini
series of we're going to do
709
:some toxicology mini series.
710
:We're going to do a mini series
on animal sexual abuse, looking
711
:at the human side, the animal side
and and some screening techniques.
712
:So we have a lot of good stuff
planned, and it's always going
713
:to be a little different.
714
:And our speakers are
experts in their field.
715
:So, um, our, our luminol and blood, uh,
sorry, our chemical enhancement for blood,
716
:um, that's going to be given by Dr.
717
:Sharon Plotkin, and, and she is an expert.
718
:Um, and she teaches all this stuff
to, uh, at a local college and
719
:she's been doing this for decades.
720
:So, you know, we always try to find
like the best person or people,
721
:um, to present these topics.
722
:DrG: Yeah, I really, I really enjoy it.
723
:And I think that as you mentioned, like
the short time frame makes it easy.
724
:Um, most of what I do is spay neuter.
725
:So I'm traveling to locations
and we usually get to a location
726
:around that time, around 9 a.
727
:m.
728
:So while we're setting it up, I'm
just listening and trying to watch
729
:while we get everything ready.
730
:So then by the time we're the talk is
over and we can, and we can move on.
731
:But yeah, I, I really.
732
:I just really enjoyed the interaction.
733
:A lot of associations don't have that
right like they they offer support
734
:and they have articles and that kind
of stuff, but it doesn't feel like
735
:that continuous flow of information.
736
:So, so yeah, please,
please keep that up because
737
:Dr. Stern: we have, I think, 27 talks
planned for the rest of the year so
738
:like there's there's a ton coming out.
739
:So we're just confirming all the speakers
before we release the massive schedule.
740
:DrG: Excellent.
741
:And what is the website for that?
742
:Dr. Stern: It's isafs.org.
743
:DrG: And then you also have
a conference coming soon.
744
:So do you want to let
people know about that?
745
:Dr. Stern: Yeah, so we have the
Animal Forensic Sciences, uh, Animal
746
:Forensic Investigations Conference.
747
:Sorry, it's sponsored by our
lab, the Veterinary Forensic
748
:Sciences, uh, Laboratory.
749
:And it is May 20th, 22nd of this year.
750
:It's in sunny Gainesville, Florida.
751
:Um, We're close to the beach.
752
:We're not at the beach.
753
:Um, but we have a three day event
and it's really covering all
754
:aspects of the investigation.
755
:Um, from the investigation itself.
756
:I think it's about a day's worth of
content for the actual investigation,
757
:um, to sort of specific types of animals.
758
:So we have a small animal mini sessions.
759
:We have a large animal, we have wildlife.
760
:Um, and then we have a couple
of talks on like preparing
761
:for court and search warrants.
762
:Uh, so we really cover everything from
the beginning of the investigation,
763
:uh, to the courts in the end.
764
:Um, so we're really excited about that.
765
:Uh, it's a great lineup of speakers and
they all, they all are very knowledgeable.
766
:DrG: Yeah, I'm excited about it.
767
:I'm excited that it's in Florida.
768
:So, um, as of right now, I'm
planning on attending because I
769
:think it's a really great lineup.
770
:Well,
771
:Dr. Stern: you have great, you have
great speakers to pull aside and have
772
:little sessions with if you want.
773
:DrG: Oh, oh, I'm planning on that too.
774
:So you may want, you may want all
your participants that I'm going
775
:to be hitting them up if I show up.
776
:Dr. Stern: Absolutely.
777
:We'll, we'll gladly have you.
778
:DrG: Excellent.
779
:So is there anything that
I've forgotten to cover or
780
:anything that you wanted to add?
781
:Dr. Stern: No, I think I, I really
enjoyed this chit chat in the morning.
782
:Um, I think, you know, I just
want everybody to know that
783
:we're here to, to help them.
784
:Um, you know, I always say, And there's
no, there's no silly question, right?
785
:Um, you can always ask a question
and you may, or you may get an
786
:answer of, Oh, that's super easy.
787
:Or no, no one's ever thought about
that, but it is a really good
788
:question or something in the middle.
789
:Um, but don't be afraid to ask questions.
790
:Um, tell my students that all the time.
791
:I tell everybody when I lecture,
it's like, just stop me.
792
:Just ask me a question.
793
:I'd rather, I'd rather be able to talk
to you than talking sort of at you.
794
:Um, when I give a seminar and things
like that, but ask questions, we're,
795
:we're absolutely here to, to help.
796
:And, you know, we might not be
able to help you, but we can, you
797
:know, maybe find somebody who can.
798
:Um, but there are those cases where,
you know, unfortunately, like the,
799
:the answer that you want, you just,
you just can't get it, but, uh,
800
:reach out to us and we'll totally
chat and figure out what's going on.
801
:DrG: And that makes sense, right?
802
:Because that's what we're about.
803
:We're about answering questions.
804
:It's just the nature of what we do.
805
:Dr. Stern: That's right.
806
:The biggest question I get asked
is, is, you know, why dead, right?
807
:Um, you know, for entomology,
it's, it's how long dead?
808
:Uh, you know, so, so you
just ask a question and we
809
:try to come up with a plan.
810
:DrG: Excellent.
811
:Well, thank you so much for taking
the time for being here, to be here.
812
:Uh, I've really enjoyed this conversation.
813
:I think it's a great start to our To,
to getting more of these episodes out.
814
:So, uh, this has been great.
815
:I hope that, that the listeners have
learned and anything that people have
816
:not gotten, we've given them resources
and websites to, to follow up so
817
:that they can get more information.
818
:So I hope you have a great day with
the rest of your day and to everybody
819
:that's listening thank you for
listening and thank you for caring.